Description
Allows the analysis of paramagnetic species, mainly metal ion centres and free radicals;
Enables the structural elucidation of metal-based systems and the characterization of radical scavenging entities or mechanisms involving the production of free radicals.
EQUIPMENT: Bruker ELEXYS III E580
- Operates in X-band (9.5 GHz) and Q-band (35 GHz) frequencies;
- Designed for liquid and solid samples;
- Equipped with variable temperature units (77 - 400K);
- For identification and characterization of species with a variable number of unpaired electrons;
- Provides information about the electronic structure of the paramagnetic species and the neighbour nucleus.
EXPERIMENTAL DETAILS
- Samples for analysis must be in the liquid phase or solid phase. The solid phase generally requires magnetic dilution, unless the sample paramagnetic species is naturally diluted;
- The solutions must be preferentially prepared with solvents of low dielectric coefficient and previously air degassed to avoid contamination with molecular oxygen. The concentration of sample that is needed depends on the nature of the species of interest, and typically is from 10-3 to 10-5 M;
- Typical sample volume used for X-band is about 400 µL and the samples are analysed in quartz tubes (3mm of diameter and 140 mm length). The nature of the solvent may require the use of tubes of smaller volume. Samples can also be analysed in capillary tube (50 µL volume required);
- Spin trapping studies can be performed to study free radicals’ production.
